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monoclonal mouse antibody to ipla2β  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology monoclonal mouse antibody to ipla2β
Fig. 1 | Cardiac phenotypes of cardiac-specific <t>iPLA2β-deficient</t> mice after pressure overload. The Pla2g6+/+ and Pla2g6–/– mice were subjected to transverse aortic constriction (TAC) and then analyzed 5 days after the operation. a Survival ratio after TAC. n = 17 (sham-operated Pla2g6+/+), 13 (sham-operated Pla2g6–/–), 51 (TAC-operated Pla2g6+/+), and 51 (TAC-operated Pla2g6–/–). The log-rank test was used for survival analysis. Sham Pla2g6+/+ versus TAC Pla2g6+/+ P = 0.0000001, TAC Pla2g6+/+ versus TAC Pla2g6–/– P = 0.0002. b Representative images of transthoracic M-mode echocardiographic tracing (scale bars, 0.2 s and 5 mm, respectively) and the echocardiographic parameters of the mice. n = 7 (sham-operated Pla2g6+/+), 7 (sham-operated Pla2g6–/–), 14 (TAC-operated Pla2g6+/+) and 10 (TAC-operated Pla2g6–/–). LVIDd and LVIDs, end-diastolic and end-systolic left ventricular (LV) internal dimensions; FS, fractional shortening of LV. c Physiological parameters of the mice. n = 7 (sham-operated Pla2g6+/+), 7 (sham-operated Pla2g6–/–), 14 (TAC- operated Pla2g6+/+), and 10 (TAC-operated Pla2g6–/–), biologically independent samples. d Representative images of the hematoxylin—eosin-stained heart sections. Experiment was repeated five times independently with similar results. Scale bar,
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polyclonal rabbit antibody to ipla2δ  (Proteintech)
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Proteintech polyclonal rabbit antibody to ipla2δ
Fig. 1 | Cardiac phenotypes of cardiac-specific <t>iPLA2β-deficient</t> mice after pressure overload. The Pla2g6+/+ and Pla2g6–/– mice were subjected to transverse aortic constriction (TAC) and then analyzed 5 days after the operation. a Survival ratio after TAC. n = 17 (sham-operated Pla2g6+/+), 13 (sham-operated Pla2g6–/–), 51 (TAC-operated Pla2g6+/+), and 51 (TAC-operated Pla2g6–/–). The log-rank test was used for survival analysis. Sham Pla2g6+/+ versus TAC Pla2g6+/+ P = 0.0000001, TAC Pla2g6+/+ versus TAC Pla2g6–/– P = 0.0002. b Representative images of transthoracic M-mode echocardiographic tracing (scale bars, 0.2 s and 5 mm, respectively) and the echocardiographic parameters of the mice. n = 7 (sham-operated Pla2g6+/+), 7 (sham-operated Pla2g6–/–), 14 (TAC-operated Pla2g6+/+) and 10 (TAC-operated Pla2g6–/–). LVIDd and LVIDs, end-diastolic and end-systolic left ventricular (LV) internal dimensions; FS, fractional shortening of LV. c Physiological parameters of the mice. n = 7 (sham-operated Pla2g6+/+), 7 (sham-operated Pla2g6–/–), 14 (TAC- operated Pla2g6+/+), and 10 (TAC-operated Pla2g6–/–), biologically independent samples. d Representative images of the hematoxylin—eosin-stained heart sections. Experiment was repeated five times independently with similar results. Scale bar,
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Image Search Results


Fig. 1 | Cardiac phenotypes of cardiac-specific iPLA2β-deficient mice after pressure overload. The Pla2g6+/+ and Pla2g6–/– mice were subjected to transverse aortic constriction (TAC) and then analyzed 5 days after the operation. a Survival ratio after TAC. n = 17 (sham-operated Pla2g6+/+), 13 (sham-operated Pla2g6–/–), 51 (TAC-operated Pla2g6+/+), and 51 (TAC-operated Pla2g6–/–). The log-rank test was used for survival analysis. Sham Pla2g6+/+ versus TAC Pla2g6+/+ P = 0.0000001, TAC Pla2g6+/+ versus TAC Pla2g6–/– P = 0.0002. b Representative images of transthoracic M-mode echocardiographic tracing (scale bars, 0.2 s and 5 mm, respectively) and the echocardiographic parameters of the mice. n = 7 (sham-operated Pla2g6+/+), 7 (sham-operated Pla2g6–/–), 14 (TAC-operated Pla2g6+/+) and 10 (TAC-operated Pla2g6–/–). LVIDd and LVIDs, end-diastolic and end-systolic left ventricular (LV) internal dimensions; FS, fractional shortening of LV. c Physiological parameters of the mice. n = 7 (sham-operated Pla2g6+/+), 7 (sham-operated Pla2g6–/–), 14 (TAC- operated Pla2g6+/+), and 10 (TAC-operated Pla2g6–/–), biologically independent samples. d Representative images of the hematoxylin—eosin-stained heart sections. Experiment was repeated five times independently with similar results. Scale bar,

Journal: Nature communications

Article Title: Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34.

doi: 10.1038/s41467-023-40201-4

Figure Lengend Snippet: Fig. 1 | Cardiac phenotypes of cardiac-specific iPLA2β-deficient mice after pressure overload. The Pla2g6+/+ and Pla2g6–/– mice were subjected to transverse aortic constriction (TAC) and then analyzed 5 days after the operation. a Survival ratio after TAC. n = 17 (sham-operated Pla2g6+/+), 13 (sham-operated Pla2g6–/–), 51 (TAC-operated Pla2g6+/+), and 51 (TAC-operated Pla2g6–/–). The log-rank test was used for survival analysis. Sham Pla2g6+/+ versus TAC Pla2g6+/+ P = 0.0000001, TAC Pla2g6+/+ versus TAC Pla2g6–/– P = 0.0002. b Representative images of transthoracic M-mode echocardiographic tracing (scale bars, 0.2 s and 5 mm, respectively) and the echocardiographic parameters of the mice. n = 7 (sham-operated Pla2g6+/+), 7 (sham-operated Pla2g6–/–), 14 (TAC-operated Pla2g6+/+) and 10 (TAC-operated Pla2g6–/–). LVIDd and LVIDs, end-diastolic and end-systolic left ventricular (LV) internal dimensions; FS, fractional shortening of LV. c Physiological parameters of the mice. n = 7 (sham-operated Pla2g6+/+), 7 (sham-operated Pla2g6–/–), 14 (TAC- operated Pla2g6+/+), and 10 (TAC-operated Pla2g6–/–), biologically independent samples. d Representative images of the hematoxylin—eosin-stained heart sections. Experiment was repeated five times independently with similar results. Scale bar,

Article Snippet: The following antibodies were used in this study: a monoclonal mouse antibody to iPLA2β (sc376563, Santa Cruz Biotechnology, Product Clone Name; D-4, 1:100 dilution), a monoclonal mouse antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (016-25523, FUJIFILM Wako Pure Chemical Corporation, Product Clone Name; 5A12, 1:1000 dilution), a monoclonal mouse antibody to iPLA2ζ (MAB3210, Abnova, Product Clone Name; AT2G2, 1:1000dilution), a polyclonal goat antibody to iPLA2ε (EB08402, Everest Biotech, 1:1000 dilution), a polyclonal rabbit antibody to iPLA2η (25469-1-AP, Protein Tech, 1:500 dilution), a polyclonal rabbit antibody to iPLA2δ (NBP1-74214, Nobus Bio, 1:500 dilution), a polyclonal rabbit antibody to iPLA2γ (PA5-49993, Thermo Fisher Scientific, 1:1000 dilution), a polyclonal rabbit antibody to GPR132 (17026-1-AP, Protein Tech, 1:1000 dilution), a polyclonal rabbit antibody to GPR34 (PA5-45717, Thermo Fisher Scientific, 1:1000 dilution), a monoclonal rabbit antibody to RIP1 (#3493, Cell Signaling Technology, Product Clone Name; D94C12, 1:1000 dilution), a polyclonal rabbit antibody to phospho-RIP1 (#31122, Cell Signaling Technology, 1:1000 dilution), a polyclonal rabbit antibody to RIP3 (NBP1-77299, Nobus Bio, 1:1000 dilution), and a monoclonal rabbit antibody to phospho-RIP3 (ab195117, Abcam, Product Clone Name; EPR9516(N)−25, 1:1000 dilution).

Techniques: Staining